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cd16 32  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cd16 32
    Cd16 32, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd16/CD16%2FCD32+(2%2E4G2)+Rat+mAb/pm41405281-272-11-12
    Average 94 stars, based on 15 article reviews
    cd16 32 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: SARS-CoV-2 Infection of Microglia Elicits Proinflammatory Activation and Apoptotic Cell Death.
    Article Snippet: Cells were lysed in radioimmunoprecipitation (RIPA) buffer (Thermo Fisher Scientific), and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). .. The membrane was incubated with 5% skim milk (BD Biosciences) in tris-buffered saline with 0.1% Tween 20 (TBST) buffer and the primary antibodies, namely, anti-SARS-CoV-2 NP (40143-R001, Sino biological), anti-CD68 (sc-17832, Santa Cruz Biotechnology) anti-GSDMDC1 (sc-81868, Santa Cruz Biotechnology), anti-Actin (sc-47778, Santa Cruz Biotechnology), anti-Hsp70 (sc-24, Santa Cruz Biotechnology), anti-CX3CL1 (ab25088, Abcam), anti-CX3CR1 (ab8021, Abcam), anti-CD16 (80006S, Cell Signaling Technology), anti-phospho-Stat1 (9167S, Cell Signaling Technology), anti-Stat1 (14994S, Cell Signaling Technology), anti-Fas (4233T, Cell Signaling Technology), antiDR4 (42533T, Cell Signaling Technology), anti-DR5 (8074T, Cell Signaling Technology), anti-TNFR2 (3727T, Cell Signaling Technology), anti-Bcl-2 (4223T, Cell Signaling Technology), anti-Bim (2933T, Cell Signaling Technology), anti-Bid (2002T, Cell Signaling Technology), anti-Bax (5023T, Cell Signaling Technology), anti-caspase-9 (9502S, Cell Signaling Technology), anti-caspase-8 (9746S, Cell Signaling Technology), anti-caspase-3 (9665S, Cell Signaling Technology), anti-caspase-1 (3866S, Cell Signaling Technology), antiNLRP3 (15101S, Cell Signaling Technology), and anti-PARP (9542S, Cell Signaling Technology). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies from Bio-Rad and ECL reagents (Thermo Fisher Scientific) were used for protein detection.

    Incubation:

    Article Title: SARS-CoV-2 Infection of Microglia Elicits Proinflammatory Activation and Apoptotic Cell Death.
    Article Snippet: Cells were lysed in radioimmunoprecipitation (RIPA) buffer (Thermo Fisher Scientific), and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). .. The membrane was incubated with 5% skim milk (BD Biosciences) in tris-buffered saline with 0.1% Tween 20 (TBST) buffer and the primary antibodies, namely, anti-SARS-CoV-2 NP (40143-R001, Sino biological), anti-CD68 (sc-17832, Santa Cruz Biotechnology) anti-GSDMDC1 (sc-81868, Santa Cruz Biotechnology), anti-Actin (sc-47778, Santa Cruz Biotechnology), anti-Hsp70 (sc-24, Santa Cruz Biotechnology), anti-CX3CL1 (ab25088, Abcam), anti-CX3CR1 (ab8021, Abcam), anti-CD16 (80006S, Cell Signaling Technology), anti-phospho-Stat1 (9167S, Cell Signaling Technology), anti-Stat1 (14994S, Cell Signaling Technology), anti-Fas (4233T, Cell Signaling Technology), antiDR4 (42533T, Cell Signaling Technology), anti-DR5 (8074T, Cell Signaling Technology), anti-TNFR2 (3727T, Cell Signaling Technology), anti-Bcl-2 (4223T, Cell Signaling Technology), anti-Bim (2933T, Cell Signaling Technology), anti-Bid (2002T, Cell Signaling Technology), anti-Bax (5023T, Cell Signaling Technology), anti-caspase-9 (9502S, Cell Signaling Technology), anti-caspase-8 (9746S, Cell Signaling Technology), anti-caspase-3 (9665S, Cell Signaling Technology), anti-caspase-1 (3866S, Cell Signaling Technology), antiNLRP3 (15101S, Cell Signaling Technology), and anti-PARP (9542S, Cell Signaling Technology). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies from Bio-Rad and ECL reagents (Thermo Fisher Scientific) were used for protein detection.

    Saline:

    Article Title: SARS-CoV-2 Infection of Microglia Elicits Proinflammatory Activation and Apoptotic Cell Death.
    Article Snippet: Cells were lysed in radioimmunoprecipitation (RIPA) buffer (Thermo Fisher Scientific), and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). .. The membrane was incubated with 5% skim milk (BD Biosciences) in tris-buffered saline with 0.1% Tween 20 (TBST) buffer and the primary antibodies, namely, anti-SARS-CoV-2 NP (40143-R001, Sino biological), anti-CD68 (sc-17832, Santa Cruz Biotechnology) anti-GSDMDC1 (sc-81868, Santa Cruz Biotechnology), anti-Actin (sc-47778, Santa Cruz Biotechnology), anti-Hsp70 (sc-24, Santa Cruz Biotechnology), anti-CX3CL1 (ab25088, Abcam), anti-CX3CR1 (ab8021, Abcam), anti-CD16 (80006S, Cell Signaling Technology), anti-phospho-Stat1 (9167S, Cell Signaling Technology), anti-Stat1 (14994S, Cell Signaling Technology), anti-Fas (4233T, Cell Signaling Technology), antiDR4 (42533T, Cell Signaling Technology), anti-DR5 (8074T, Cell Signaling Technology), anti-TNFR2 (3727T, Cell Signaling Technology), anti-Bcl-2 (4223T, Cell Signaling Technology), anti-Bim (2933T, Cell Signaling Technology), anti-Bid (2002T, Cell Signaling Technology), anti-Bax (5023T, Cell Signaling Technology), anti-caspase-9 (9502S, Cell Signaling Technology), anti-caspase-8 (9746S, Cell Signaling Technology), anti-caspase-3 (9665S, Cell Signaling Technology), anti-caspase-1 (3866S, Cell Signaling Technology), antiNLRP3 (15101S, Cell Signaling Technology), and anti-PARP (9542S, Cell Signaling Technology). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies from Bio-Rad and ECL reagents (Thermo Fisher Scientific) were used for protein detection.

    Staining:

    Article Title: Exploration of Immune-Modulatory Effects of Amivantamab in Combination with Pembrolizumab in Lung and Head and Neck Squamous Cell Carcinoma
    Article Snippet: .. Multiple staining rounds were performed using the following antibodies: anti-CD68 (76437, Cell Signaling Technology, Massachusetts, US), anti-PD-L1 (ACI 3171A, Cell Signaling Technology), anti-granzyme B (Cell Signaling Technology), anti-CD16 (24326, Cell Signaling Technology), anti-CD163 (NCL-L-CD163, Leica Biosystems), anti-CD11c (45581, Cell Signaling Technology), anti-CD4 (ab181724, Abcam, Cambridge, United Kingdom), anti-CD8 (CD8-4B11-L-CE, Leica Biosystems), anti-PD-1 (ab137132, Abcam) anti-FoxP3 (98377; Cell Signaling Technology), anti-PanCK (AE1/AE3-601-L-CE; Leica Biosystems). .. Tissue sections were counterstained with Spectral 4′,6-diamidino-2-phenylindole (FP1490; Akoya Biosciences).

    Conjugation Assay:

    Article Title: NetTracer3D Enables User-Friendly Analysis of Diverse Microscopic and Medical 3D Datasets
    Article Snippet: The slide was stained with the PhenoCodeTM Discovery IO60 panel (Quanterix) of sixty DNA-barcoded antibodies, excluding antibodies against Keratin 14 and CD21. .. In addition, DNA-barcoded antibodies anti-HIF1alpha (Cat. 4550069, Quanterix) and anti-NKp46 (clone 29A1.4, gift of Sean Tracey and Michael Farrar, University of Minnesota) were included alongside the following antibodies which had been individually barcoded using an antibody conjugation kit (Cat. 7000009, Quanterix): Anti-CD45RA (Cat. No. 304102, Biolegend), anti-CD16 (Cat. No. 72204SF, Cell Signaling Technology), anti-CD7 (Cat. No. ab230834, Abcam), anti-CD49a (Cat. No. 328302, Biolegend), anti-CD69 (Cat. No. ab234512, Abcam), anti-Na+/K+ ATPase (Cat. No. ab167390, Abcam), anti-CD33 (Cat. No. MAB11371, R&D systems), anti-B7H3 (Cat. No. 58798SF, Cell Signaling Technologies) and anti-PSMA (Cat. No. 92728SF, Cell Signaling Technologies). .. Imaging was performed at 20x magnification on a PhenoImager (Quanterix).



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    Image Search Results


    Flow immunophenotyping release testing data are presented in A-G and IFNγ ELISpot data presented in H-K (Arm A: n = 12; Arms B/C: n = 27). Each data point represents a separate TAA-T cell product infused into patients (biological replicates). Lower whisker is defined as the minima, upper whisker is defined as the maxima, center is defined as median, lower bound of box is defined as Q1 (25th percentile), and upper bound of box is defined as Q3 (75th percentile). a ) % Total T cells (CD3 + ). Arm A: Minima=51, Maxima=99, Median=97, 25th Percentile=95, 75th Percentile=99; Arms B/C: Minima=31, Maxima=100, Median=97, 25th Percentile=94, 75th Percentile=98. b ) % CD4 + T cells (CD3 + CD4+ of CD45 + ). Arm A: Minima=0.50, Maxima=88, Median=16, 25th Percentile=2.6, 75th Percentile=38; Arms B/C: Minima=0.40, Maxima=55, Median=9.9, 25th Percentile=6.1, 75th Percentile=17. c ) %CD8 + T cells (% CD3 + CD8+ of CD45 + ). Arm A: Minima=4.3, Maxima=88, Median=37, 25th Percentile=22, 75th Percentile=47; Arms B/C: Minima=19, Maxima=91, Median=51, 25th Percentile=36, 75th Percentile=71. d ) % αβ T cells (% TCRαβ+ of CD3 + ). Arm A: Minima=8.2, Maxima=94, Median=69, 25th Percentile=28, 75th Percentile=86; Arms B/C: Minima=32, Maxima=98, Median=71, 25th Percentile=48, 75th Percentile=84. e ) % γδ T cells (% TCRγδ+ of CD3 + ). Arm A: Minima=5.1, Maxima=79, Median=28, 25th Percentile=8.4, 75th Percentile=67; Arms B/C: Minima=1.1, Maxima=83, Median=25, 25th Percentile=10, 75th Percentile=53. f ) % CD3 + CD16 + CD56+ of CD45 + . Arm A: Minima=2.8, Maxima=25, Median=11, 25th Percentile=7.2, 75th Percentile=17; Arms B/C: Minima=3.4, Maxima=64, Median=16, 25th Percentile=8.1, 75th Percentile=28. g ) % NK cells (CD16/CD56 + CD3- of CD45 + ). Arm A: Minima=0.40, Maxima=48, Median=1.0, 25th Percentile=0.48, 75th Percentile=4.6; Arms B/C: Minima=0.10, Maxima=62, Median=1.6, 25th Percentile=0.60, 75th Percentile=6.1. h - i ) IFNγ ELISpot with background correction for Arm A ( h ) and Arms B/C ( i ). Negative values after background normalization have been normalized to zero for visual representation.

    Journal: Nature Medicine

    Article Title: Multi-antigen-targeting T cells in pediatric central nervous system tumors: a phase 1 trial

    doi: 10.1038/s41591-026-04449-9

    Figure Lengend Snippet: Flow immunophenotyping release testing data are presented in A-G and IFNγ ELISpot data presented in H-K (Arm A: n = 12; Arms B/C: n = 27). Each data point represents a separate TAA-T cell product infused into patients (biological replicates). Lower whisker is defined as the minima, upper whisker is defined as the maxima, center is defined as median, lower bound of box is defined as Q1 (25th percentile), and upper bound of box is defined as Q3 (75th percentile). a ) % Total T cells (CD3 + ). Arm A: Minima=51, Maxima=99, Median=97, 25th Percentile=95, 75th Percentile=99; Arms B/C: Minima=31, Maxima=100, Median=97, 25th Percentile=94, 75th Percentile=98. b ) % CD4 + T cells (CD3 + CD4+ of CD45 + ). Arm A: Minima=0.50, Maxima=88, Median=16, 25th Percentile=2.6, 75th Percentile=38; Arms B/C: Minima=0.40, Maxima=55, Median=9.9, 25th Percentile=6.1, 75th Percentile=17. c ) %CD8 + T cells (% CD3 + CD8+ of CD45 + ). Arm A: Minima=4.3, Maxima=88, Median=37, 25th Percentile=22, 75th Percentile=47; Arms B/C: Minima=19, Maxima=91, Median=51, 25th Percentile=36, 75th Percentile=71. d ) % αβ T cells (% TCRαβ+ of CD3 + ). Arm A: Minima=8.2, Maxima=94, Median=69, 25th Percentile=28, 75th Percentile=86; Arms B/C: Minima=32, Maxima=98, Median=71, 25th Percentile=48, 75th Percentile=84. e ) % γδ T cells (% TCRγδ+ of CD3 + ). Arm A: Minima=5.1, Maxima=79, Median=28, 25th Percentile=8.4, 75th Percentile=67; Arms B/C: Minima=1.1, Maxima=83, Median=25, 25th Percentile=10, 75th Percentile=53. f ) % CD3 + CD16 + CD56+ of CD45 + . Arm A: Minima=2.8, Maxima=25, Median=11, 25th Percentile=7.2, 75th Percentile=17; Arms B/C: Minima=3.4, Maxima=64, Median=16, 25th Percentile=8.1, 75th Percentile=28. g ) % NK cells (CD16/CD56 + CD3- of CD45 + ). Arm A: Minima=0.40, Maxima=48, Median=1.0, 25th Percentile=0.48, 75th Percentile=4.6; Arms B/C: Minima=0.10, Maxima=62, Median=1.6, 25th Percentile=0.60, 75th Percentile=6.1. h - i ) IFNγ ELISpot with background correction for Arm A ( h ) and Arms B/C ( i ). Negative values after background normalization have been normalized to zero for visual representation.

    Article Snippet: Antibodies for the T/NK panel included CD8 FITC (Miltenyi Biotec, cat. no. 130-110-677), CD16 PE (Miltenyi Biotec, cat. no. 130-113-393), CD56 PE (Miltenyi Biotec, cat. no. 130-113-312), CD3 Per CP Vio-770 (Miltenyi Biotec, cat. no. 130-113-141), CD4 PE-Vio-770 (Miltenyi Biotec, cat. no. 130-113-227) and CD45 APC (Miltenyi Biotec, cat. no. 130-110-633).

    Techniques: Enzyme-linked Immunospot, Whisker Assay